  {"id":131594,"date":"2020-12-02T09:11:11","date_gmt":"2020-12-02T19:11:11","guid":{"rendered":"https:\/\/www.hawaii.edu\/news\/?p=131594"},"modified":"2020-12-02T09:11:11","modified_gmt":"2020-12-02T19:11:11","slug":"rapid-test-detect-bacterial-wilt","status":"publish","type":"post","link":"https:\/\/www.hawaii.edu\/news\/2020\/12\/02\/rapid-test-detect-bacterial-wilt\/","title":{"rendered":"<abbr>UH<\/abbr> develop rapid test to detect bacterial wilt in Guam"},"content":{"rendered":"<span class=\"span-reading-time rt-reading-time\" style=\"display: block;\"><span class=\"rt-label rt-prefix\">Reading time: <\/span> <span class=\"rt-time\"> &lt; 1<\/span> <span class=\"rt-label rt-postfix\">minute<\/span><\/span><figure id=\"attachment_130002\" aria-describedby=\"caption-attachment-130002\" style=\"width: 676px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.hawaii.edu\/news\/wp-content\/uploads\/2020\/11\/manoa-ctahr-guamwilt.jpg\" alt=\"group of people\" width=\"676\" height=\"381\" class=\"size-full wp-image-130002\" srcset=\"https:\/\/www.hawaii.edu\/news\/wp-content\/uploads\/2020\/11\/manoa-ctahr-guamwilt.jpg 676w, https:\/\/www.hawaii.edu\/news\/wp-content\/uploads\/2020\/11\/manoa-ctahr-guamwilt-300x169.jpg 300w, https:\/\/www.hawaii.edu\/news\/wp-content\/uploads\/2020\/11\/manoa-ctahr-guamwilt-130x73.jpg 130w\" sizes=\"auto, (max-width: 676px) 100vw, 676px\" \/><figcaption id=\"caption-attachment-130002\" class=\"wp-caption-text\">Grad students Dario Arizala and Sujan Paudel with Assistant Researcher\/Assistant Professor Mohammad Arif in the <abbr>CTAHR<\/abbr><abbr> greenhouse.<\/abbr><\/figcaption><\/figure>\n<p>Bacteria wilt is a problem affecting numerous trees in Guam. Under a grant from the United States Department of Agriculture (<abbr>USDA<\/abbr>), a University of <span aria-label=\"Hawaii\">Âé¶¹´«Ã½<\/span> at M\u0101noa assistant researcher and graduate students have helped to develop tests to rapidly distinguish the bacterial strain attacking the plants. <\/p>\n<p>The <abbr>USDA<\/abbr>\u2019s Priority Pest List for 2021 includes <em>Ralstonia solanacearum race 3 biovar 2<\/em>, a bacterium better known as a bacterial wilt. It infects through the roots and is deadly to plants, and is the subject of new grant funding for the <a href=\"https:\/\/www.uog.edu\/\">University of Guam<\/a> under the <abbr>USDA<\/abbr>\u2019s Plant Protection Act.<\/p>\n<p>Assisting with the characterization of Guam\u2019s bacterial wilt strains are <strong>Mohammad Arif<\/strong> of <abbr title=\"University of Hawaii\">UH<\/abbr> M\u0101noa\u2019s College of Tropical Agriculture and Human Resources <a href=\"https:\/\/cms.ctahr.hawaii.edu\/PEPS\">Department of Plant and Environmental Protection Sciences<\/a> and graduate students <strong>Sujan Paudel<\/strong>, <strong>Dario Arizala<\/strong> and <strong>Diksha Klair<\/strong>. Paudel and Shefali Dobhal recently developed rapid assay tests that can accurately and quickly distinguish the race 3 biovar 2 strain from the <em>R. solanacearum<\/em> species complex. <\/p>\n<p>&ldquo;With these diagnostic assays in hand, we can rapidly detect the bacteria directly from crude host tissue sap. We are now responsible for understanding how bacteria interact inside the host tissues, as well as mapping out endophytic communities associated with ironwood decline through microbiome studies,&rdquo; said Arif. <\/p>\n<p>&ldquo;We\u2019ll also study genetic variability among <em>Ralstonia <\/em>strains found associated with ironwood decline, and how this bacteria has evolved,&rdquo; he added. &ldquo;These objectives will enhance our understanding of this pathogen and disease, toward the development of effective disease-management strategies.&rdquo;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The rapid assay tests can accurately and quickly distinguish the race 3 biovar 2 strain from the <em>R. solanacearum<\/em> species complex.<\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[30],"tags":[1363,359,9],"class_list":["post-131594","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-research","tag-manoa-research","tag-plant-and-environmental-protection-sciences","tag-uh-manoa","entry","has-media"],"aioseo_notices":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"https:\/\/www.hawaii.edu\/news\/wp-json\/wp\/v2\/posts\/131594","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.hawaii.edu\/news\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.hawaii.edu\/news\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.hawaii.edu\/news\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.hawaii.edu\/news\/wp-json\/wp\/v2\/comments?post=131594"}],"version-history":[{"count":8,"href":"https:\/\/www.hawaii.edu\/news\/wp-json\/wp\/v2\/posts\/131594\/revisions"}],"predecessor-version":[{"id":131606,"href":"https:\/\/www.hawaii.edu\/news\/wp-json\/wp\/v2\/posts\/131594\/revisions\/131606"}],"wp:attachment":[{"href":"https:\/\/www.hawaii.edu\/news\/wp-json\/wp\/v2\/media?parent=131594"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.hawaii.edu\/news\/wp-json\/wp\/v2\/categories?post=131594"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.hawaii.edu\/news\/wp-json\/wp\/v2\/tags?post=131594"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}