{"id":10,"date":"2014-11-07T00:26:53","date_gmt":"2014-11-07T00:26:53","guid":{"rendered":"http:\/\/www.hawaii.edu\/microbiology\/asgpb\/?page_id=10"},"modified":"2022-04-21T23:05:12","modified_gmt":"2022-04-21T23:05:12","slug":"services","status":"publish","type":"page","link":"https:\/\/www.hawaii.edu\/microbiology\/asgpb\/?page_id=10","title":{"rendered":""},"content":{"rendered":"<p><a href=\"http:\/\/www.hawaii.edu\/microbiology\/asgpb\/wp-content\/uploads\/2014\/11\/servicesheader.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-92 size-full\" src=\"http:\/\/www.hawaii.edu\/microbiology\/asgpb\/wp-content\/uploads\/2014\/11\/servicesheader.png\" alt=\"servicesheader\" width=\"1100\" height=\"200\" srcset=\"https:\/\/www.hawaii.edu\/microbiology\/asgpb\/wp-content\/uploads\/2014\/11\/servicesheader.png 1100w, https:\/\/www.hawaii.edu\/microbiology\/asgpb\/wp-content\/uploads\/2014\/11\/servicesheader-300x54.png 300w, https:\/\/www.hawaii.edu\/microbiology\/asgpb\/wp-content\/uploads\/2014\/11\/servicesheader-1024x186.png 1024w, https:\/\/www.hawaii.edu\/microbiology\/asgpb\/wp-content\/uploads\/2014\/11\/servicesheader-250x45.png 250w, https:\/\/www.hawaii.edu\/microbiology\/asgpb\/wp-content\/uploads\/2014\/11\/servicesheader-150x27.png 150w\" sizes=\"auto, (max-width: 1100px) 100vw, 1100px\" \/><\/a><\/p>\n<h1 style=\"text-align: center;\"><\/h1>\n<h1 style=\"text-align: center;\"><\/h1>\n<div class=\"wc-shortcodes-row wc-shortcodes-item wc-shortcodes-clearfix\">\n<div class=\"wc-shortcodes-column wc-shortcodes-content wc-shortcodes-one-third wc-shortcodes-column-first \">\n<p style=\"text-align: right;\"><strong>Sanger DNA Sequencing<\/strong><\/p>\n<p style=\"text-align: left;\"><\/div>\n<div class=\"wc-shortcodes-column wc-shortcodes-content wc-shortcodes-two-third wc-shortcodes-column-last \">\n<p style=\"text-align: left;\">Plasmid DNA or PCR products undergo a cycle sequencing reaction (BigDye terminator chemistry), cleanup, and capillary electrophoresis in an Applied Biosystems 3730XL DNA Analyzer. The sequences produced have read lengths up to 1000 bases.<\/p>\n<hr \/>\n<\/div>\n<\/div>\n<div class=\"wc-shortcodes-row wc-shortcodes-item wc-shortcodes-clearfix\">\n<div class=\"wc-shortcodes-column wc-shortcodes-content wc-shortcodes-one-third wc-shortcodes-column-first \">\n<p style=\"text-align: right;\"><strong>Genotyping<\/strong><\/p>\n<\/div>\n<div class=\"wc-shortcodes-column wc-shortcodes-content wc-shortcodes-two-third wc-shortcodes-column-last \">\n<p>User-prepared fluorescently-labeled DNA fragments are mixed with the appropriate size standards and subjected to capillary electrophoresis in an Applied Biosystems 3730XL DNA Analyzer. The fragment profiles are provided to the user.<\/p>\n<hr \/>\n<\/div>\n<\/div>\n<div class=\"wc-shortcodes-row wc-shortcodes-item wc-shortcodes-clearfix\">\n<div class=\"wc-shortcodes-column wc-shortcodes-content wc-shortcodes-one-third wc-shortcodes-column-first \">\n<p style=\"text-align: right;\"><strong>Illumina MiSeq Sequencing<\/strong><\/p>\n<\/div>\n<div class=\"wc-shortcodes-column wc-shortcodes-content wc-shortcodes-two-third wc-shortcodes-column-last \">\n<p>Libraries are constructed from DNA or RNA samples. The libraries are sequenced using a MiSeq system, with each run producing up to 15 Gb (billion bases) of data. Data output will depend on the application, read length, and amount of data required by the user.<\/p>\n<hr \/>\n<\/div>\n<\/div>\n<div class=\"wc-shortcodes-row wc-shortcodes-item wc-shortcodes-clearfix\">\n<div class=\"wc-shortcodes-column wc-shortcodes-content wc-shortcodes-one-third wc-shortcodes-column-first \">\n<p style=\"text-align: right;\"><strong>IDT Oligo Synthesis<\/strong><\/p>\n<\/div>\n<div class=\"wc-shortcodes-column wc-shortcodes-content wc-shortcodes-two-third wc-shortcodes-column-last \">\n<p>Order oligonucleotides and other products from the Integrated DNA Technologies \u2013 ASGPB Core Portal.<\/p>\n<hr \/>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Sanger DNA Sequencing Plasmid DNA or PCR products undergo a cycle sequencing reaction (BigDye terminator chemistry), cleanup, and capillary electrophoresis in an Applied Biosystems 3730XL DNA Analyzer. The sequences produced have read lengths up to 1000 bases. Genotyping User-prepared fluorescently-labeled DNA fragments are mixed with the appropriate size standards and\u2026<\/p>\n<p> <a class=\"continue-reading-link\" href=\"https:\/\/www.hawaii.edu\/microbiology\/asgpb\/?page_id=10\"><span>Continue reading<\/span><i class=\"crycon-right-dir\"><\/i><\/a> <\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"templates\/template-onecolumn.php","meta":{"footnotes":""},"class_list":["post-10","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.hawaii.edu\/microbiology\/asgpb\/index.php?rest_route=\/wp\/v2\/pages\/10","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.hawaii.edu\/microbiology\/asgpb\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.hawaii.edu\/microbiology\/asgpb\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.hawaii.edu\/microbiology\/asgpb\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.hawaii.edu\/microbiology\/asgpb\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=10"}],"version-history":[{"count":33,"href":"https:\/\/www.hawaii.edu\/microbiology\/asgpb\/index.php?rest_route=\/wp\/v2\/pages\/10\/revisions"}],"predecessor-version":[{"id":613,"href":"https:\/\/www.hawaii.edu\/microbiology\/asgpb\/index.php?rest_route=\/wp\/v2\/pages\/10\/revisions\/613"}],"wp:attachment":[{"href":"https:\/\/www.hawaii.edu\/microbiology\/asgpb\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=10"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}